Background: This study was conducted out to assess the function of trace elements in oral submucous fibrosis subjects.

Objectives: To assess the association between serum ferritin levels and hypothyroidism, evaluate gender differences, and compare ferritin in subclinical and overt hypothyroidism.

Material and methods: A total of 100 participants were involved in the study, consisting of 50 controls and 50 individuals diagnosed with OSMF. Each patient was confirmed to have OSMF through histological examination. Blood samples from 100 unique individuals were analyzed using a computerized autoanalyzer photometer. This colorimetric analysis aimed to assess the copper levels in the blood. Statistical methods were utilized to compare the results between the case group and the control group.

Results: In this study, there were 50 subjects in control group and 50 subjects in group 2. The concentrations of copper had been discovered to be lesser in the control group as well as greater in the study group. The mean serum copper concentrations in the individuals of study group and control group were 209.6±1.2 μg/ml and 102.5±042 μg/ml, respectively.

Conclusion: The most accurate method for diagnosing Oral Submucous Fibrosis (OSMF) is via biopsy; however, this approach is the most invasive and demands significant time. A variety of contemporary advancements are being employed to detect OSMF at an early stage and avert its advancement to severe and irreversible stages. Analyzing trace elements may act as a valuable diagnostic and prognostic instrument for patients suffering from oral submucous fibrosis (OSMF).